Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 (CLIon Channel1) is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.1) Novarino, G., et al., (2004) J. Neurosci. 24 (23), 5322-5330.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLIon Channel1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CLIon Channel1 antibody can be used for detection of CLIon Channel1 by ELISA at 1:62500. CLIon Channel1 antibody can be used for detection of CLIon Channel1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10420 - Fetal Small Intestine Tissue Lysate
Application Notes
CLIon Channel1 antibody can be used for detection of CLIon Channel1 by ELISA at 1:62500. CLIon Channel1 antibody can be used for detection of CLIon Channel1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CLIon Channel1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.