Western blot
Host: Rabbit Target Name: ASIC1 Sample Tissue: MCF7 cell lysates Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: ACCN2 Sample Tissue: Human Adult Placenta Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: ACCN2 Sample Tissue: Human Fetal Muscle Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: ACCN2 Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: ACCN2 Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: ACCN2 Sample Tissue: Human Fetal Heart Antibody Dilution: 1.0ug/mlWestern blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HT1080 cell lysate| Product Name | Rabbit anti-ACCN2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASIC, ASIC1A, BNaC2, hBNaC2, ACCN2 |
| Immunogen | The immunogen for anti-ACCN2 antibody: synthetic peptide directed towards the N terminal of human ACCN2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACCN2 |
|---|---|
| Gene Synonyms | ASIC ASIC1A BNaC2 hBNaC2 ACCN2 |
| Gene Full Name | Acid-sensing (proton-gated) ion channel 1 |
| Gene Summary | ACCN2 is the cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ACCN2 is also permeable for Ca2+, Li+ and K+. ACCN2 generates a biphasic current with a fast inactivating and a slow sustained phase. ACCN2 mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. ACCN2 functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. It modulates activity in the circuits underlying innate fear.This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products. |
| Alternative Names | ASIC ASIC1A BNaC2 hBNaC2 ACCN2 |
| Molecular Weight(MW) | 65kDa |
| Sequence | 574 amino acids |
Western blot
Host: Rabbit Target Name: ASIC1 Sample Tissue: MCF7 cell lysates Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: ACCN2 Sample Tissue: Human Adult Placenta Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: ACCN2 Sample Tissue: Human Fetal Muscle Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: ACCN2 Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: ACCN2 Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: ACCN2 Sample Tissue: Human Fetal Heart Antibody Dilution: 1.0ug/ml
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HT1080 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
