Immunoblot
1: 60ug rat shwann cell, 2: 60ug human melanoma (YUMAC), 3: 60ug human melanoma (SK-MEL5), 4: 60ug human melanocytes (HFSC), 5: 60ug mouse melanoma (B16), 6: 60ug mouse melanocytes (melba)Primary Antibody Dilution: 1:200Secondary Antibody: Donkey anti-rabbit HPRTSecondary Antibody Dilution: 1:2000Gene Name: SMARCD3Submitted by: Dr. Ivana de la Serna, University of ToledoWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate| Product Name | Rabbit anti-SMARCD3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BAF60C, CRACD3, MGC111010, Rsc6p |
| Immunogen | The immunogen for anti-SMARCD3 antibody: synthetic peptide directed towards the N terminal of human SMARCD3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanPigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMARCD3 |
|---|---|
| Gene Synonyms | BAF60C CRACD3 MGC111010 Rsc6p |
| Gene Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 |
| Gene Summary | SMARCD3 is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCD3 is part of the lar. |
| Alternative Names | BAF60C CRACD3 MGC111010 Rsc6p |
| Molecular Weight(MW) | 55kDa |
| Sequence | 483 amino acids |
| Entrez Gene | 6604 |
|---|---|
| SwissProt ID | Q6STE5 |
| Protein Accession | NP_001003801 |
Immunoblot
1: 60ug rat shwann cell, 2: 60ug human melanoma (YUMAC), 3: 60ug human melanoma (SK-MEL5), 4: 60ug human melanocytes (HFSC), 5: 60ug mouse melanoma (B16), 6: 60ug mouse melanocytes (melba)Primary Antibody Dilution: 1:200Secondary Antibody: Donkey anti-rabbit HPRTSecondary Antibody Dilution: 1:2000Gene Name: SMARCD3Submitted by: Dr. Ivana de la Serna, University of Toledo
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
