Western blot
Sample Type: 1. Human RPE cell line (15ug)2. Bovine retina lysate cleared at 100,000Xg for 2 hours (15ug)Primary Dilution: 1:1000Secondary Anditbody: goat anti-rabbit HRPScondary Dilution: 1:10,000Image Submitted By: Gerald LiewStanford University| Product Name | Rabbit anti-IFT140 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp564L232, FLJ10306, FLJ30571, KIAA0590, WDTC2, c305C8.4, c380F5.1, gs114 |
| Immunogen | The immunogen for anti-IFT140 antibody: synthetic peptide directed towards the N terminal of human IFT140 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseMousePigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFT140 |
|---|---|
| Gene Synonyms | DKFZp564L232 FLJ10306 FLJ30571 KIAA0590 WDTC2 c305C8.4 c380F5.1 gs114 |
| Gene Full Name | Intraflagellar transport 140 homolog (Chlamydomonas) |
| Gene Summary | IFT140 contains 9 TPR repeats and 5 WD repeats. The function of the IFT140 protein remains unknown. |
| Alternative Names | DKFZp564L232 FLJ10306 FLJ30571 KIAA0590 WDTC2 c305C8.4 c380F5.1 gs114 |
| Molecular Weight(MW) | 165kDa |
| Sequence | 1462 amino acids |
Western blot
Sample Type: 1. Human RPE cell line (15ug)2. Bovine retina lysate cleared at 100,000Xg for 2 hours (15ug)Primary Dilution: 1:1000Secondary Anditbody: goat anti-rabbit HRPScondary Dilution: 1:10,000Image Submitted By: Gerald LiewStanford University| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
