Western blot
Positive Control: Lane1: 100ug mouse liver, Lane2: 100ug mouse brain, Lane3: 100ug mouse heart, Lane4: 100ug mouse kidney, Lane5: 100ug mouse lung, Lane6: 100ug mouse thymus, Lane7: 100ug mouse spleen, Lane8: 100ug mouse testis, Lane9: 100ug mouse HeLa Primary Antibody Dilution : 1:1000Secondary Antibody : Anti-rabbit-AP Secondry Antibody Dilution : 1:10,000Submitted by: Andreia Carvalho, Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)
Western blot
0.2-1 ug/mlPositive Control: Mouse Lung