Western blot
0.2-1 ug/mlPositive Control: Mouse HeartImmunoblot
30ug NIH3T3 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1: 5000Gene Name: Ttf1Submitted by: Thomas Moss| Product Name | Rabbit anti-Ttf1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AV245725 |
| Immunogen | The immunogen for anti-Ttf1 antibody: synthetic peptide directed towards the n terminal of mouse Ttf1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Ttf1 |
|---|---|
| Gene Synonyms | AV245725 |
| Gene Full Name | Transcription termination factor, RNA polymerase I |
| Gene Summary | Ttf1 is a multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin.Ttf1 plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription.Ttf1 interaction with BAZ2A/TIP5 recovers DNA-binding activity. |
| Alternative Names | AV245725 |
| Molecular Weight(MW) | 98 |
| Sequence | 859 amino acids |
Western blot
0.2-1 ug/mlPositive Control: Mouse Heart
Immunoblot
30ug NIH3T3 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1: 5000Gene Name: Ttf1Submitted by: Thomas Moss| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
