Immunofluorescence
organ of CortiWB
(A) Representative immunoblot against ER stress-related proteins from hepatocytes treated with 300 μM of MCT for 6, 12, 24, 36, and 48 h.(E) Representative immunoblot ER stress-related apoptosis-related proteins from hepatocytes treated with different doses of MCT (200, 300, and 400 μM) for 36 h.WB
(C) Detection of ER stress-related proteins, including GRP78, IRE1 α, p-IRE1 α, ATF6, eIF2 α, p-eIF2 α, ATF4, and CHOP by western blot. (D–F) Quantitative analysis of protein levels in C.WB
(A) Western blots analysis of Grp78, PERK, phospho-PERK (Thr980), eIF2α, phospho-eIF2α(Ser51), ATF4, and CHOP.WB
(a) Expression of hepatocyte PERK signaling pathway-related proteins was analyzed by Western blotting.| Product Name | Rabbit anti-Atf4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-Atf4 antibody: synthetic peptide corresponding to a region of Mouse |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| WB:1:500~1:2000 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMousePigRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Atf4 |
|---|---|
| Gene Synonyms | Atf-4 C/ATF CREB2 MGC96460 TAXREB67 |
| Gene Full Name | Activating transcription factor 4 |
| Gene Summary | The function of this protein remains unknown. |
| Alternative Names | Atf-4 C/ATF CREB2 MGC96460 TAXREB67 |
| Molecular Weight(MW) | 38kDa |
| Sequence | 349 amino acids |
Immunofluorescence
organ of Corti
WB
(A) Representative immunoblot against ER stress-related proteins from hepatocytes treated with 300 μM of MCT for 6, 12, 24, 36, and 48 h.(E) Representative immunoblot ER stress-related apoptosis-related proteins from hepatocytes treated with different doses of MCT (200, 300, and 400 μM) for 36 h.
WB
(C) Detection of ER stress-related proteins, including GRP78, IRE1 α, p-IRE1 α, ATF6, eIF2 α, p-eIF2 α, ATF4, and CHOP by western blot. (D–F) Quantitative analysis of protein levels in C.
WB
(A) Western blots analysis of Grp78, PERK, phospho-PERK (Thr980), eIF2α, phospho-eIF2α(Ser51), ATF4, and CHOP.
WB
(a) Expression of hepatocyte PERK signaling pathway-related proteins was analyzed by Western blotting.| Application Notes | WB:1:500~1:2000 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
