WB
WB analysis of HEK-293 using ATF4 Monoclonal antibody. HEK-293 cells were subjected to SDS PAGE followed by western blot with ATF4 Antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.WB
WB analysis of human placenta using ATF4 Monoclonal antibody. human placenta tissue were subjected to SDS PAGE followed by western blot with ATF4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human breast cancer using ATF4 Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using ATF4 Antibody at dilution of 1:200 (under 10x lens).ICC/IF
IF Staining of A549 using ATF4 Monoclonal antibody. Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ATF4 antibody at dilution of 1:400 and 488-Conjugated Goat Anti-Mouse IgG(H+L).FC
FC experiment of HeLa using ATF4 Monoclonal antibody. 1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF4 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Product Name | ATF4 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ATF4 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ATF4 |
|---|---|
| Gene Synonyms | CREB2 TXREB CREB-2 TAXREB67 |
| Gene Full Name | activating transcription factor 4 |
| Gene Summary | This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011] |
| Alternative Names | 3E4C5 cAMP-dependent transcription factor ATF-4 CREB2 Cyclic AMP-responsive element-binding protein 2 Tax-responsive enhancer element-binding protein 67 |
| Molecular Weight(MW) | 39 kDa(Observed band size: 45-50 kDa) |
| Cellular Localization | Cell membrane,Cytoplasm,Cytoskeleton,Membrane,Nucleus. |
WB
WB analysis of HEK-293 using ATF4 Monoclonal antibody. HEK-293 cells were subjected to SDS PAGE followed by western blot with ATF4 Antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB
WB analysis of human placenta using ATF4 Monoclonal antibody. human placenta tissue were subjected to SDS PAGE followed by western blot with ATF4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human breast cancer using ATF4 Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using ATF4 Antibody at dilution of 1:200 (under 10x lens).
ICC/IF
IF Staining of A549 using ATF4 Monoclonal antibody. Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ATF4 antibody at dilution of 1:400 and 488-Conjugated Goat Anti-Mouse IgG(H+L).
FC
FC experiment of HeLa using ATF4 Monoclonal antibody. 1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF4 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
