Western blot
Positive Control: Lane 1: 50ug mouse glomerular endothelial lysate Lane 2: 50ug mouse glomerular endothelial lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:5000 Submitted by: Xiaoxin Wang, UC DenveWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: SP2/0 cell lysate| Product Name | Rabbit anti-SREBF1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADD1, ADD-1, SREBP1, bHLHd1, SREBP-1, SREBP1c, SREBP-1a, SREBP-1c, D630008H06 |
| Immunogen | The immunogen for anti-SREBF1 antibody: synthetic peptide directed towards the N terminal of mouse SREBF1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SREBF1 |
|---|---|
| Gene Synonyms | ADD1 ADD-1 SREBP1 bHLHd1 SREBP-1 SREBP1c SREBP-1a SREBP-1c D630008H06 |
| Gene Full Name | Sterol regulatory element binding transcription factor 1 |
| Gene Summary | Srebf1 is transcriptional activator that binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Srebf1 also binds to an E-box motif (5'-ATCACGTGA-3'). Srebf1 regulates the transcription of genes for sterol biosynthesis and the LDL receptor gene. |
| Alternative Names | ADD1 ADD-1 SREBP1 bHLHd1 SREBP-1 SREBP1c SREBP-1a SREBP-1c D630008H06 |
| Molecular Weight(MW) | 125kDa |
| Sequence | 1134 amino acids |
Western blot
Positive Control: Lane 1: 50ug mouse glomerular endothelial lysate Lane 2: 50ug mouse glomerular endothelial lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:5000 Submitted by: Xiaoxin Wang, UC Denve
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: SP2/0 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
