WB
(A) Western blot analysis of SREBP-1c, SCD1, FASN, ACC, and ACL.IF
(A) Immunofluorescence staining was conducted to determine the effect of FGF21 or BML-275 on SREBF1 nuclear translocation in hepatocytes (left). Green, SREBF1 staining; blue, DAPI nuclear staining (630×). Fluorescent intensity from 4 randomly selected microscopic fields per group (right).| Product Name | Rabbit anti-SREBF1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-SREBF1 antibody: synthetic peptide directed towards the middle region of human SREBF1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Species Reactivity | BovineGoatHorseHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SREBF1 |
|---|---|
| Gene Synonyms | SREBP1 bHLHd1 SREBP-1c |
| Gene Full Name | Sterol regulatory element binding transcription factor 1 |
| Gene Summary | SREBF1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family.This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | SREBP1 bHLHd1 SREBP-1c |
| Molecular Weight(MW) | 125kDa |
| Sequence | 1177 amino acids |
| Entrez Gene | 6720 |
|---|---|
| SwissProt ID | P36956 |
| Protein Accession | NP_001005291 |
WB
(A) Western blot analysis of SREBP-1c, SCD1, FASN, ACC, and ACL.
IF
(A) Immunofluorescence staining was conducted to determine the effect of FGF21 or BML-275 on SREBF1 nuclear translocation in hepatocytes (left). Green, SREBF1 staining; blue, DAPI nuclear staining (630×). Fluorescent intensity from 4 randomly selected microscopic fields per group (right).| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
