Immunohistochemical
500 ug rat brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :G3BP1Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :G3BP1Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunoprecipitation
Positive Control: NT2 CELL/BRAIN TISSUEImmunoblot
1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: G3BP1Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science| Product Name | Rabbit anti-G3BP1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G3BP, HDH-VIII, MGC111040 |
| Immunogen | The immunogen for anti-G3BP1 antibody: synthetic peptide directed towards the N terminal of human G3BP1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | G3BP1 |
|---|---|
| Gene Synonyms | G3BP HDH-VIII MGC111040 |
| Gene Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
| Gene Summary | This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding |
| Alternative Names | G3BP HDH-VIII MGC111040 |
| Molecular Weight(MW) | 52kDa |
| Sequence | 466 amino acids |
Immunohistochemical
500 ug rat brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :G3BP1Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :G3BP1Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunoprecipitation
Positive Control: NT2 CELL/BRAIN TISSUE
Immunoblot
1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: G3BP1Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
