Western blot
Host: Rabbit Target Name:ID4 Sample Tissue:HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%| Product Name | Rabbit anti-ID4 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IDB4, bHLHb27 |
| Immunogen | The immunogen for anti-ID4 antibody: synthetic peptide directed towards the N terminal of human ID4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ID4 |
|---|---|
| Gene Synonyms | IDB4 bHLHb27 |
| Gene Full Name | Inhibitor of DNA binding 4, dominant negative helix-loop-helix protein |
| Gene Summary | Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995).[supplied by OMIM]. |
| Alternative Names | IDB4 bHLHb27 |
| Molecular Weight(MW) | 17kDa |
| Sequence | 161 amino acids |
Western blot
Host: Rabbit Target Name:ID4 Sample Tissue:HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
