Application
Antibody used in WB on Human HEK293T cells at 0.2-1 ug/ml.Application
Antibody used in WB on MDA-MB231, MCF7 at 1:1000 (Lane 1: 10ug MDA-MB231 lysate, Lane 2: 10ug MCF7 lysate ).| Product Name | ID4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ID4, IDB4, bHLHb27 |
| Product description | Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins.Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Russell, L.J., (2008) Blood 111 (1), 387-391. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ID4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ID4 antibody can be used for detection of ID4 by ELISA at 1:62500. ID4 antibody can be used for detection of ID4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ID4 |
|---|---|
| Alternative Names | ID4 IDB4 bHLHb27 |
| Molecular Weight(MW) | 16 kDa |
Application
Antibody used in WB on Human HEK293T cells at 0.2-1 ug/ml.
Application
Antibody used in WB on MDA-MB231, MCF7 at 1:1000 (Lane 1: 10ug MDA-MB231 lysate, Lane 2: 10ug MCF7 lysate ).| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | ID4 antibody can be used for detection of ID4 by ELISA at 1:62500. ID4 antibody can be used for detection of ID4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ID4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
