Immunohistochemistry
Immunohistochemistry with Brain, cerebellum tissue at an antibody concentration of 5ug/ml using anti-E2F2 antibodyWestern blot
0.2-1 ug/ml| Product Name | E2F2 antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-E2F2 antibody: synthetic peptide directed towards the middle region of human E2F2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IHC |
| Species Reactivity | Bovine,Dog,Guinea Pig,Horse,Human,Mouse,Rabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E2F2 |
|---|---|
| Gene Synonyms | E2F-2 |
| Gene Full Name | E2F transcription factor 2 |
| Gene Summary | E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | E2F-2 |
| Molecular Weight(MW) | 47kDa |
| Sequence | 437 amino acids |
Immunohistochemistry
Immunohistochemistry with Brain, cerebellum tissue at an antibody concentration of 5ug/ml using anti-E2F2 antibody
Western blot
0.2-1 ug/ml| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
