Application
Western blot analysis of lysed extracts from HeLa and K562 cells using E2F-2 Antibody.| Product Name | E2F2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2F-2, HER2, MLN19, NEU, NGL, E2F-2 |
| Product description | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.1) Rubin S. M., Gall A. -L., Zheng N., Pavletich N. P. Cell 123:1093-1106 (2005) |
| Immunogen | E2F-2 antibody was raised against a peptide sequence around aa.425~429 (L-F-D-S-Y) derived from Human E2F-2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E2F2 |
|---|---|
| Alternative Names | E2F-2 HER2 MLN19 NEU NGL E2F-2 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | This antibody detects endogenous level of total E2F-2 protein. |
| Entrez Gene | 1870 |
|---|---|
| Protein Accession | NP_004082.1 |
Application
Western blot analysis of lysed extracts from HeLa and K562 cells using E2F-2 Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
