Western blot
Host: Rabbit Target Name:TARDBP Sample Tissue:JurkatLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:1.25ug/mL Peptide Concentration: 1.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%| Product Name | Rabbit anti-TARDBP polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALS10, TDP-43 |
| Immunogen | The immunogen for anti-TARDBP antibody: synthetic peptide directed towards the C terminal of human TARDBP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TARDBP |
|---|---|
| Gene Synonyms | ALS10 TDP-43 |
| Gene Full Name | TAR DNA binding protein |
| Gene Summary | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. |
| Alternative Names | ALS10 TDP-43 |
| Molecular Weight(MW) | 45kDa |
| Sequence | 414 amino acids |
Western blot
Host: Rabbit Target Name:TARDBP Sample Tissue:JurkatLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:1.25ug/mL Peptide Concentration: 1.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
