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GAPDH antibody - N-terminal region

Catalog Number:OM109621
OM109621
Discount Price
OM109621-100ul
1~2 Week
100ul
OM109621-50ul
50ul
OM109621-20ul
20ul
1~2 Week
Product Profile
Product Name GAPDH antibody - N-terminal region
Antibody Type Primary Antibodies
Immunogen The immunogen for anti-GAPDH antibody: synthetic peptide directed towards the N terminal of human GAPDH
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WB
Species Reactivity Bovine,Dog,Guinea Pig,Horse,Human,Mouse,Pig,Rabbit,Rat,Sheep
Concentration 1mg/ml
Purification Affinity purified
Target Information
Gene Symbol GAPDH
Gene Synonyms G3PD
GAPD
MGC88685
Gene Full Name Glyceraldehyde-3-phosphate dehydrogenase
Gene Summary GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alternative Names G3PD
GAPD
MGC88685
Molecular Weight(MW) 36kDa
Sequence 335 amino acids
Database Links
Entrez Gene 2597
SwissProt ID Q2TSD0
Protein Accession NP_002037
Application

Western blot

0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human brain

Immunoblot

1. 50 ug NIH3T3 cytosolic extract 2. 50 ug NIH3T3 cytosolic extract 3. 50 ug NIH3T3 cytosolic extract 4. 50 ug NIH3T3 cytosolic extract
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:30000
Gene Name:
GAPDH
Submitted by:
Dr. Arimdam Basu, Dept. of Animal Biology, University of Pennsylvania

Western blot

Host: Rabbit
Target Name: GAPDH
Sample Tissue: Hela Whole Cell lysates
Antibody Dilution: 1.0ug/ml
Additional Information
Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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