Western blot
How many ug’s of tissue/cell lysate run on the gel: 20 ugWhat species+tissue/cell type run on the gel: HEK293Primary antibody dilution: 1 to 1000Secondary antibody: IRD800CW (anti-rabbit) (LICOR)Secondary antibody dilution: 1 to 20000Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human kidneyImmunoblot
1) 10 ug 2) 20 ug 3) 40 ug U87 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: HRP-goat anti-rabbit IgG(H+L) antibodySecondary Antibody Dilution: 1: 15000Gene Name: GAPDHSubmitted by: Prof. Varda Shushan-Barmatz, Ben-Gurion University| Product Name | Rabbit anti-GAPDH polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G3PD, GAPD, MGC88685 |
| Immunogen | The immunogen for anti-GAPDH antibody: synthetic peptide directed towards the middle region of human GAPDH |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAPDH |
|---|---|
| Gene Synonyms | G3PD GAPD MGC88685 |
| Gene Full Name | Glyceraldehyde-3-phosphate dehydrogenase |
| Gene Summary | GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a |
| Alternative Names | G3PD GAPD MGC88685 |
| Molecular Weight(MW) | 36kDa |
| Sequence | 335 amino acids |
Western blot
How many ug’s of tissue/cell lysate run on the gel: 20 ugWhat species+tissue/cell type run on the gel: HEK293Primary antibody dilution: 1 to 1000Secondary antibody: IRD800CW (anti-rabbit) (LICOR)Secondary antibody dilution: 1 to 20000
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human kidney
Immunoblot
1) 10 ug 2) 20 ug 3) 40 ug U87 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: HRP-goat anti-rabbit IgG(H+L) antibodySecondary Antibody Dilution: 1: 15000Gene Name: GAPDHSubmitted by: Prof. Varda Shushan-Barmatz, Ben-Gurion University| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
