Immunohistochemical
Mouse brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :NSF: Red DAPI:BlueGene Name :NSFSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
Sample Type: 1. NT-2 cells2. mouse brain extractsPrimary Antibody Dilution: 2ug/mlSecondary Antibody Dilution: 1: 20,000Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceLane 1: 60ug/laneLane 2: 80ug/laneImage Submitted by: Yuzhi ChenUniversity of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlPositive Control: Human brain| Product Name | Rabbit anti-NSF polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SKD2 |
| Immunogen | The immunogen for anti-NSF antibody: synthetic peptide directed towards the C terminal of human NSF |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NSF |
|---|---|
| Gene Synonyms | SKD2 |
| Gene Full Name | N-ethylmaleimide-sensitive factor |
| Gene Summary | The functions of NSF remain unknown. |
| Alternative Names | SKD2 |
| Molecular Weight(MW) | 83kDa |
| Sequence | 744 amino acids |
| Entrez Gene | 4905 |
|---|---|
| SwissProt ID | P46459 |
| Protein Accession | XP_001128468 |
Immunohistochemical
Mouse brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :NSF: Red DAPI:BlueGene Name :NSFSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
Sample Type: 1. NT-2 cells2. mouse brain extractsPrimary Antibody Dilution: 2ug/mlSecondary Antibody Dilution: 1: 20,000Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceLane 1: 60ug/laneLane 2: 80ug/laneImage Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science
Western blot
0.2-1 ug/mlPositive Control: Human brain| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
