Application
Western blot (10% SDS-PAGE) of 3T3 (lane 1), PC12 (lane 2), MDBK (lane 3) and normal human fibroblasts (lane 4) probed with clone NSF-1 anti-NSF at 0.5 µg/ml. The diffuse bands at ~70 kDa were also present on a blot incubated with second antibody alone (data not shown).| Product Name | NSF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [NSF - 1] to NSFN ethylmaleimide sensitive factor (NSF) is an ~83 kDa protein (predicted MW) which was initially isolated based on its ability to restore intercisternal Golgi transport to N ethylmaleimide treated membranes. NSF was subsequently discovered to be involved in a variety of membrane fusion events. NSF is an ATPase with two ATP binding domains that are essential for membrane fusion and homo oligomerization. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). NSF has been shown to play a prominent role in synaptic vesicle fusion. Together with synaptotagmin and a SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin, in an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | NSF-1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| IF: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 76 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | SKD2 |
|---|---|
| Target | NSF |
| Cellular Localization | Cytoplasmic |
Application
Western blot (10% SDS-PAGE) of 3T3 (lane 1), PC12 (lane 2), MDBK (lane 3) and normal human fibroblasts (lane 4) probed with clone NSF-1 anti-NSF at 0.5 µg/ml. The diffuse bands at ~70 kDa were also present on a blot incubated with second antibody alone (data not shown).| Positive Control | Rat PC12 |
|---|---|
| Application Notes | IF: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 76 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None Constituents: 50% Glycerol, 20mM Sodium phosphate, 150mM Sodium chloride. pH 7.5 |
