Immunofluorescence
subnuclear bodies-paraspecklesImmunohistochemical
ImmunohistochemicalWestern blot
1.25ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-NONO polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P54, NMT55, NRB54, P54NRB |
| Immunogen | The immunogen for anti-NONO antibody: synthetic peptide directed towards the C terminal of human NONO |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500~1:2000 IHC:1:50~1:200 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NONO |
|---|---|
| Gene Synonyms | P54 NMT55 NRB54 P54NRB |
| Gene Full Name | Non-POU domain containing, octamer-binding |
| Gene Summary | NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. |
| Alternative Names | P54 NMT55 NRB54 P54NRB |
| Molecular Weight(MW) | 52kDa |
| Sequence | 471 amino acids |
Immunofluorescence
subnuclear bodies-paraspeckles
Immunohistochemical
Immunohistochemical
Western blot
1.25ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
