WB
Western blot analysis of p54nrb/NONO on different lysates with Rabbit anti-p54nrb/NONO antibody at 1/1,000 dilution. Lane 1: A549-si NT cell lysate, Lane 2: A549-si p54nrb/NONO cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-p54nrb antibody. Counter stained with hematoxylin.ICC/IF
ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | p54nrb/NONO Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human p54nrb aa 365-471 / 471. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NONO |
|---|---|
| Gene Synonyms | P54 NMT55 NRB54 MRXS34 P54NRB PPP1R114 |
| Gene Full Name | non-POU domain containing octamer binding |
| Gene Summary | This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009] |
| Alternative Names | 52 kDa subunit antibody 54 kDa nuclear RNA and DNA binding protein antibody 54 kDa nuclear RNA- and DNA-binding protein antibody 55 kDa nuclear protein antibody DNA binding p52/p100 complex 52 kDa subunit antibody DNA-binding p52/p100 complex antibody NMT 55 antibody NMT55 antibody Non Pou domain containing octamer (ATGCAAAT) binding protein antibody Non POU domain containing octamer binding antibody 52 kDa subunit antibody 54 kDa nuclear RNA and DNA binding protein antibody 54 kDa nuclear RNA- and DNA-binding protein antibody 55 kDa nuclear protein antibody DNA binding p52/p100 complex 52 kDa subunit antibody DNA-binding p52/p100 complex antibody NMT 55 antibody NMT55 antibody Non Pou domain containing octamer (ATGCAAAT) binding protein antibody Non POU domain containing octamer binding antibody Non POU domain containing octamer binding protein antibody Non-POU domain-containing octamer-binding protein antibody Nono antibody NonO protein antibody NONO_HUMAN antibody NRB 54 antibody NRB antibody NRB54 antibody Nuclear RNA binding protein 54kD antibody P54 antibody p54(nrb) antibody p54nrb antibody PPP1R114 antibody Protein phosphatase 1 regulatory subunit 114 antibody |
| Molecular Weight(MW) | 54kDa(Observed band size: 60kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of p54nrb/NONO on different lysates with Rabbit anti-p54nrb/NONO antibody at 1/1,000 dilution. Lane 1: A549-si NT cell lysate, Lane 2: A549-si p54nrb/NONO cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-p54nrb antibody. Counter stained with hematoxylin.
ICC/IF
ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
