Immunoblot
Lane 1: 7ug HeLa cystosolic lysateLane 2: 7ug HeLa nuclear lysatePrimary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: NXF3Submitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto UniversityWestern blot
0.2-1 ug/mlPositive Control: HepG2 cell lysate| Product Name | Rabbit anti-NXF3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-NXF3 antibody: synthetic peptide directed towards the N terminal of human NXF3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NXF3 |
|---|---|
| Gene Synonyms | - |
| Gene Full Name | Nuclear RNA export factor 3 |
| Gene Summary | NXF3 is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. NXF3 has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus. |
| Molecular Weight(MW) | 60kDa |
| Sequence | 531 amino acids |
Immunoblot
Lane 1: 7ug HeLa cystosolic lysateLane 2: 7ug HeLa nuclear lysatePrimary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: NXF3Submitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University
Western blot
0.2-1 ug/mlPositive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
