Application
Antibody used in WB on Human HeLa at: 1:300 (Lane 1: 7ug HeLa cystosolic lysate, Lane 2: 7ug HeLa nuclear lysate).Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | NXF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NXF3, TAPL3 |
| Product description | NXF3 is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. NXF3 has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus.1) Jun, L., (2001) Curr. Biol. 11 (18), 1381-1391. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NXF3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NXF3 antibody can be used for detection of NXF3 by ELISA at 1:312500. NXF3 antibody can be used for detection of NXF3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NXF3 |
|---|---|
| Alternative Names | NXF3 TAPL3 |
| Molecular Weight(MW) | 60 kDa |
Application
Antibody used in WB on Human HeLa at: 1:300 (Lane 1: 7ug HeLa cystosolic lysate, Lane 2: 7ug HeLa nuclear lysate).
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NXF3 antibody can be used for detection of NXF3 by ELISA at 1:312500. NXF3 antibody can be used for detection of NXF3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NXF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
