Western blot
2.5ug/mlPositive Control: Jurkat cell lysateImmunoblot
Lane 1: 7ug HeLa cystosolic extract Lane 2: 7ug HeLa nuclear extractPrimary Antibody Dilution: 1:100Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: NXF5Submitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University| Product Name | Rabbit anti-NXF5 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-NXF5 antibody: synthetic peptide directed towards the middle region of human NXF5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NXF5 |
|---|---|
| Gene Full Name | Nuclear RNA export factor 5 |
| Gene Summary | NXF5 is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. Five transcript variants that encode different isoforms have been found for this gene. |
| Molecular Weight(MW) | 44kDa |
| Sequence | 397 amino acids |
Western blot
2.5ug/mlPositive Control: Jurkat cell lysate
Immunoblot
Lane 1: 7ug HeLa cystosolic extract Lane 2: 7ug HeLa nuclear extractPrimary Antibody Dilution: 1:100Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: NXF5Submitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM110184