Immunohistochemical
Human stomachPrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-HRPSecondary Antibody Dilution :1:5000Color/Signal Descriptions :Brown: MUC1 Blue: NucleusGene Name :MUC1Submitted by :Dr. Sara Linden, University of GothenburgImmunohistochemical
Pig stomachPrimary Antibody Dilution :1:5000 + 1:2000Secondary Antibody :Anti-rabbit-HRPSecondary Antibody Dilution :1:1000Color/Signal Descriptions :Brown: MUC1 Blue: NucleusGene Name :MUC1Submitted by :Dr. Sara Linden, University of GothenburgImmunofluorescence
NMuFG cells in ImmunohistochemistryDilutions 1:50 for the three primary antibodies and 1:100 for FITC-conjugated secondary.Western blot
0.2-1 ug/mlPositive Control: Jurkat cell lysateWestern blot
Host: Rabbit Target Name:MUC1 Sample Tissue: HepG2 Whole cell lysates Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-MUC1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD227, EMA, PEM, PUM, mucin, KL-6, MAM6, PEMT, H23AG, MUC-1, MUC-1/X, MUC1/ZD, MUC-1/SEC |
| Immunogen | The immunogen for anti-MUC1 antibody: synthetic peptide directed towards the C terminal of human MUC1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MUC1 |
|---|---|
| Gene Synonyms | CD227 EMA PEM PUM mucin KL-6 MAM6 PEMT H23AG MUC-1 MUC-1/X MUC1/ZD MUC-1/SEC |
| Gene Full Name | Mucin 1, cell surface associated |
| Gene Summary | MUC1 is a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. |
| Alternative Names | CD227 EMA PEM PUM mucin KL-6 MAM6 PEMT H23AG MUC-1 MUC-1/X MUC1/ZD MUC-1/SEC |
| Molecular Weight(MW) | 22kDa |
| Sequence | 203 amino acids |
| Entrez Gene | 4582 |
|---|---|
| SwissProt ID | Q7Z552 |
| Protein Accession | NP_001037855 |
Immunohistochemical
Human stomachPrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-HRPSecondary Antibody Dilution :1:5000Color/Signal Descriptions :Brown: MUC1 Blue: NucleusGene Name :MUC1Submitted by :Dr. Sara Linden, University of Gothenburg
Immunohistochemical
Pig stomachPrimary Antibody Dilution :1:5000 + 1:2000Secondary Antibody :Anti-rabbit-HRPSecondary Antibody Dilution :1:1000Color/Signal Descriptions :Brown: MUC1 Blue: NucleusGene Name :MUC1Submitted by :Dr. Sara Linden, University of Gothenburg
Immunofluorescence
NMuFG cells in ImmunohistochemistryDilutions 1:50 for the three primary antibodies and 1:100 for FITC-conjugated secondary.
Western blot
0.2-1 ug/mlPositive Control: Jurkat cell lysate
Western blot
Host: Rabbit Target Name:MUC1 Sample Tissue: HepG2 Whole cell lysates Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
