Application
Sample Type :Human lung adenocarcinoma cell line A549Primary Antibody Dilution :1:100Secondary Antibody :Goat anti-rabbit AlexaFluor 488Secondary Antibody Dilution :1:400Color/Signal Descriptions :MAK: Green DAPI:BlueGene Name :MAK Submitted by :Dr. Crissy Dudgeon, Ph.D., CINJApplication
Lanes: 1. 30 ug MiaPaca-2 cell lysate2. 30 ug Panc-1 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-Rabbit HRPSecondary Antibody Dilution: 1:4000Gene Name: MAKSubmitted by: Dr. Crissy Dudgeon, Ph.D., CINJApplication
WB Suggested Anti-MAK Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate| Product Name | Rabbit anti-MAK polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | dJ417M14.2, RP62 |
| Immunogen | The immunogen for anti-MAK antibody: synthetic peptide directed towards the C terminal of human MAK |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAK |
|---|---|
| Gene Synonyms | MAK |
| Gene Full Name | Male germ cell-associated kinase |
| Gene Summary | MAK is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized. |
| Alternative Names | dJ417M14.2 RP62 |
| Molecular Weight(MW) | 70kDa |
| Sequence | 623 amino acids |
Application
Sample Type :Human lung adenocarcinoma cell line A549Primary Antibody Dilution :1:100Secondary Antibody :Goat anti-rabbit AlexaFluor 488Secondary Antibody Dilution :1:400Color/Signal Descriptions :MAK: Green DAPI:BlueGene Name :MAK Submitted by :Dr. Crissy Dudgeon, Ph.D., CINJ
Application
Lanes: 1. 30 ug MiaPaca-2 cell lysate2. 30 ug Panc-1 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-Rabbit HRPSecondary Antibody Dilution: 1:4000Gene Name: MAKSubmitted by: Dr. Crissy Dudgeon, Ph.D., CINJ
Application
WB Suggested Anti-MAK Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
