Application
Western blot analysis in Y79 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of MAK using MAK Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAK gene (Lane 2).| Product Name | MAK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase MAK, Male germ cell-associated kinase, MAK |
| Product description | MAK is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.1) Xia, L., et al., J. Biol. Chem. 277(38):35422-35433 (2002). Taketo, M., et al., Genomics 19(2):397-398 (1994). Jinno, A., et al., Mol. Cell. Biol. 13(7):4146-4156 (1993). Bladt, F., et al., Differentiation 53(2):115-122 (1993). Koji, T., et al., Cell Biochem. Funct. 10(4):273-279 (1992). |
| Immunogen | This MAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 578-608 amino acids from the C-terminal region of human MAK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAK |
|---|---|
| Alternative Names | Serine/threonine-protein kinase MAK Male germ cell-associated kinase MAK |
| Molecular Weight(MW) | 71 kDa |
Application
Western blot analysis in Y79 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of MAK using MAK Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAK gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
