Immunoblot
Lane 1: 2ug MCF7-DMBT1+DoxLane 2: 2ug MCF7-DMBT1 -DoxLane 3: 2ug MCF7-Ctrl+DoxLane 4: 2ug MCF7-DMBT1 -DoxPrimary Antibody Dilution: 1:5000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10,000Gene Name: DMBT1Submitted by: Matthias Rauen, Lundbeckfonden Center of Excellence NanoCAN, University of Southern DenmarkWestern blot
0.2-1 ug/mlPositive Control: Human Stomach| Product Name | Rabbit anti-DMBT1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GP340, MGC164738, muclin |
| Immunogen | The immunogen for anti-DMBT1 antibody: synthetic peptide directed towards the N terminal of human DMBT1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DMBT1 |
|---|---|
| Gene Synonyms | GP340 MGC164738 muclin |
| Gene Full Name | Deleted in malignant brain tumors 1 |
| Gene Summary | Loss of sequences from human chromosome 10q has been associated with the progression of human cancers. The gene DMBT1 was originally isolated based on its deletion in a medulloblastoma cell line. DMBT1 is expressed with transcripts of 6.0, 7.5, and 8.0 kb in fetal lung and with one transcript of 8.0 kb in adult lung, although the 7.5 kb transcript has not been characterized. The DMBT1 protein is a glycoprotein containing multiple scavenger receptor cysteine-rich (SRCR) domains separated by SRCR-interspersed domains (SID). Transcript variant 2 (8.0 kb) has been shown to bind surfactant protein D independently of carbohydrate recognition. This indicates that DMBT1 may not be a classical tumor supressor gene, but rather play a role in the interaction of tumor cells and the immune system. |
| Alternative Names | GP340 MGC164738 muclin |
| Molecular Weight(MW) | 258kDa |
| Sequence | 2413 amino acids |
Immunoblot
Lane 1: 2ug MCF7-DMBT1+DoxLane 2: 2ug MCF7-DMBT1 -DoxLane 3: 2ug MCF7-Ctrl+DoxLane 4: 2ug MCF7-DMBT1 -DoxPrimary Antibody Dilution: 1:5000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10,000Gene Name: DMBT1Submitted by: Matthias Rauen, Lundbeckfonden Center of Excellence NanoCAN, University of Southern Denmark
Western blot
0.2-1 ug/mlPositive Control: Human Stomach| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
