Immunoblot
1: 2ug mouse SYDE1 transfected HEK293T lysate, 2: 15ug untransfected HEK293T lysate, 3: 100ug wild type mouse brain lysate, 4: 100ug SYDE1 knock-out mouse brain lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit L-chain HRPSecondary Antibody Dilution: 1:10,000Gene Name: SYDE1Submitted by: AnonymousWestern blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-SYDE1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 7h3, FLJ13511 |
| Immunogen | The immunogen for anti-SYDE1 antibody: synthetic peptide directed towards the N terminal of human SYDE1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanPigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYDE1 |
|---|---|
| Gene Synonyms | 7h3 FLJ13511 |
| Gene Full Name | Synapse defective 1, Rho GTPase, homolog 1 (C. elegans) |
| Gene Summary | SYDE1 contains 1 Rho-GAP domain. It is a GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. |
| Alternative Names | 7h3 FLJ13511 |
| Molecular Weight(MW) | 80kDa |
| Sequence | 735 amino acids |
Immunoblot
1: 2ug mouse SYDE1 transfected HEK293T lysate, 2: 15ug untransfected HEK293T lysate, 3: 100ug wild type mouse brain lysate, 4: 100ug SYDE1 knock-out mouse brain lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit L-chain HRPSecondary Antibody Dilution: 1:10,000Gene Name: SYDE1Submitted by: Anonymous
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
