Immunohistochemical
ImmunohistochemicalImmunoblot
Lane 1: 50ug HeLa lysateLane 2: 50ug 293T lysateLane 3: 50ug K562 lysateLane 4: 50ug MDA-MB-231 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: COX4I1Submitted by: David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life SciencesWestern blot
COX4I1 antibody - N-terminal region validated by WB using 1. Human liver 2. Rat liver3. Wild-type mouse liver4. AMPKa1+2-/- mouse liver 5. Human muscle6. Rat muscle7. Mouse muscle at 1:1000.Western blot
1.25ug/mlPositive Control: HepG2 cell lysate| Product Name | Rabbit anti-COX4I1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COX4, COXIV, MGC72016, COX4-1 |
| Immunogen | The immunogen for anti-COX4I1 antibody: synthetic peptide directed towards the N terminal of human COX4I1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | COX4I1 |
|---|---|
| Gene Synonyms | COX4 COXIV MGC72016 COX4-1 |
| Gene Full Name | Cytochrome c oxidase subunit IV isoform 1 |
| Gene Summary | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4I1 is the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme.Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. |
| Alternative Names | COX4 COXIV MGC72016 COX4-1 |
| Molecular Weight(MW) | 19kDa |
| Sequence | 169 amino acids |
Immunohistochemical
Immunohistochemical
Immunoblot
Lane 1: 50ug HeLa lysateLane 2: 50ug 293T lysateLane 3: 50ug K562 lysateLane 4: 50ug MDA-MB-231 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: COX4I1Submitted by: David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences
Western blot
COX4I1 antibody - N-terminal region validated by WB using 1. Human liver 2. Rat liver3. Wild-type mouse liver4. AMPKa1+2-/- mouse liver 5. Human muscle6. Rat muscle7. Mouse muscle at 1:1000.
Western blot
1.25ug/mlPositive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
