Flow Cytometry
FACS -- Sample type: Bone marrow derived monocytes from mice BV2 cellsDilution: 1ug/mLWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Mouse Thymus| Product Name | Rabbit anti-Rgs10 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2310010N19Rik, MGC129414 |
| Immunogen | The immunogen for anti-Rgs10 antibody: synthetic peptide corresponding to a region of Mouse |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFACS |
| WB:1:500~1:2000 FACS:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Rgs10 |
|---|---|
| Gene Synonyms | 2310010N19Rik MGC129414 |
| Gene Full Name | Regulator of G-protein signalling 10 |
| Gene Summary | Rgs10 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Rgs10 associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)-alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity of Rgs10 on G(z)-alpha is inhibited by palmitoylation of the G-protein. |
| Alternative Names | 2310010N19Rik MGC129414 |
| Molecular Weight(MW) | 21kDa |
| Sequence | 181 amino acids |
Flow Cytometry
FACS -- Sample type: Bone marrow derived monocytes from mice BV2 cellsDilution: 1ug/mL
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Mouse Thymus| Application Notes | WB:1:500~1:2000 FACS:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
