Immunoblot
1: 40ng HIS-UBE2D1 protein2: 40ng HIS-UBE2D2 protein3: 40ng HIS-UBE2D3 protein4: 40ng HIS-UBE2D4 protein5: 40ng HIS-UBE2E1 protein6: 40ng HIS-UBE2E2 protein7: 40ng HIS-UBE2E3 protein8: 40ng HIS-UBE2K protein9: 40ng HIS-UBE2L3 protein10: 40ng HIS-UBE2N protein11: 40ng HIS-UBE2V1 protein12: 40ng HIS-UBE2V2 protein.Primary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:50,000Gene Name: UBE2D1Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.Western blot
1.25ug/mlPositive Control: Jurkat cell lysate| Product Name | Rabbit anti-UBE2D1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A |
| Immunogen | The immunogen for anti-UBE2D1 antibody: synthetic peptide directed towards the middle region of human UBE2D1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGoatMousePigZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | UBE2D1 |
|---|---|
| Gene Synonyms | E2(17)KB1 SFT UBC4/5 UBCH5 UBCH5A |
| Gene Full Name | Ubiquitin-conjugating enzyme E2D 1 |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. |
| Alternative Names | E2(17)KB1 SFT UBC4/5 UBCH5 UBCH5A |
| Molecular Weight(MW) | 16kDa |
| Sequence | 147 amino acids |
Immunoblot
1: 40ng HIS-UBE2D1 protein2: 40ng HIS-UBE2D2 protein3: 40ng HIS-UBE2D3 protein4: 40ng HIS-UBE2D4 protein5: 40ng HIS-UBE2E1 protein6: 40ng HIS-UBE2E2 protein7: 40ng HIS-UBE2E3 protein8: 40ng HIS-UBE2K protein9: 40ng HIS-UBE2L3 protein10: 40ng HIS-UBE2N protein11: 40ng HIS-UBE2V1 protein12: 40ng HIS-UBE2V2 protein.Primary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:50,000Gene Name: UBE2D1Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.
Western blot
1.25ug/mlPositive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
