Western blot
0.2-1 ug/mlPositive Control: Jurkat cell lysateImmunohistochemistry
Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-UBE2L3 antibody| Product Name | Rabbit anti-UBE2L3 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2-F1, L-UBC, UBCH7, UbcM4 |
| Immunogen | The immunogen for anti-UBE2L3 antibody: synthetic peptide directed towards the C terminal of human UBE2L3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2L3 |
|---|---|
| Gene Synonyms | E2-F1 L-UBC UBCH7 UbcM4 |
| Gene Full Name | Ubiquitin-conjugating enzyme E2L 3 |
| Gene Summary | UBE2L3(ubiquitin-conjugating enzyme E2L 3) Antibody The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Alternative Names | E2-F1 L-UBC UBCH7 UbcM4 |
| Molecular Weight(MW) | 17kDa |
| Sequence | 154 amino acids |
Western blot
0.2-1 ug/mlPositive Control: Jurkat cell lysate
Immunohistochemistry
Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-UBE2L3 antibody| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
