Application
Antibody used in IHC on Human Prostate at 5.0 ug/ml.Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | UBE2L3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4 |
| Product description | UBE2L3 (ubiquitin-conjugating enzyme E2L 3) Antibody The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.1) Collins, J.E., Genome Biol. 5 (10), R84 (2004). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2L3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| UBE2L3 antibody can be used for detection of UBE2L3 by ELISA at 1:62500. UBE2L3 antibody can be used for detection of UBE2L3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2L3 |
|---|---|
| Alternative Names | UBE2L3 E2-F1 L-UBC UBCH7 UbcM4 |
| Molecular Weight(MW) | 17 kDa |
Application
Antibody used in IHC on Human Prostate at 5.0 ug/ml.
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | UBE2L3 antibody can be used for detection of UBE2L3 by ELISA at 1:62500. UBE2L3 antibody can be used for detection of UBE2L3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store UBE2L3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
