Western blot
Host: Rabbit Target Name: RNF139 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: RNF139 Sample Tissue: 293T Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: RNF139 Sample Tissue: Hela Antibody Dilution: 1.0ug/mlWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Product Name | Rabbit anti-RNF139 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HRCA1, MGC31961, RCA1, TRC8 |
| Immunogen | The immunogen for anti-RNF139 antibody: synthetic peptide directed towards the N terminal of human RNF139 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorsePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF139 |
|---|---|
| Gene Synonyms | HRCA1 MGC31961 RCA1 TRC8 |
| Gene Full Name | Ring finger protein 139 |
| Gene Summary | RNF139 is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. |
| Alternative Names | HRCA1 MGC31961 RCA1 TRC8 |
| Molecular Weight(MW) | 76kDa |
| Sequence | 664 amino acids |
Western blot
Host: Rabbit Target Name: RNF139 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: RNF139 Sample Tissue: 293T Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: RNF139 Sample Tissue: Hela Antibody Dilution: 1.0ug/ml
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM111201