WB
Western blot analysis of VHL using anti-VHL antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VHL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of VHL using anti-VHL antibody. VHL was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-VHL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of VHL using anti-VHL antibody. VHL was detected in a paraffin-embedded section of human kidney tissue. The tissue section was incubated with rabbit anti-VHL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of VHL using anti-VHL antibody and anti-Beta Tubulin antibody. VHL was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-VHL Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.| Product Name | Anti-VHL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human VHL recombinant protein (Position: W88-D213). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-2000 ICC/IF:1:50-400 IHC:1:50-400 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VHL |
|---|---|
| Gene Synonyms | RCA1 VHL1 pVHL HRCA1 |
| Gene Full Name | von Hippel-Lindau tumor suppressor |
| Gene Summary | This gene encodes a component of a ubiquitination complex. The encoded protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. In addition to oxygen-related gene expression, this protein plays a role in many other cellular processes including cilia formation, cytokine signaling, regulation of senescence, and formation of the extracellular matrix. Variants of this gene are associated with von Hippel-Lindau syndrome, pheochromocytoma, erythrocytosis, renal cell carcinoma, and cerebellar hemangioblastoma. [provided by RefSeq, Jun 2022] |
| Molecular Weight(MW) | 20kDa(Observed MW 18kDa) |
| Cellular Localization | Cytoplasm |
WB
Western blot analysis of VHL using anti-VHL antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VHL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of VHL using anti-VHL antibody. VHL was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-VHL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of VHL using anti-VHL antibody. VHL was detected in a paraffin-embedded section of human kidney tissue. The tissue section was incubated with rabbit anti-VHL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of VHL using anti-VHL antibody and anti-Beta Tubulin antibody. VHL was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-VHL Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.| Application Notes | WB:1:500-2000 ICC/IF:1:50-400 IHC:1:50-400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
Data sheet for OM680499