Western blot
Titration: 1.0 ug/mlPositive Control: Rat Lung| Product Name | Rabbit anti-Rnf11l polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rnf11 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | RatHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Rnf11l |
|---|---|
| Gene Synonyms | Rnf11 |
| Gene Full Name | Ring finger protein 11-like |
| Gene Summary | Rnf11l is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Rnf11l promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Rnf11l recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome. |
| Alternative Names | Rnf11 |
| Molecular Weight(MW) | 30kDa |
| Sequence | 280 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: Rat Lung| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
