Western blot
Western blotWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: PANC1 cell lysate| Product Name | Rabbit anti-NEDD4L polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ33870, KIAA0439, RSP5, hNedd4-2, NEDD4-2, NEDD4.2, hNEDD4-2 |
| Immunogen | The immunogen for anti-NEDD4L antibody: synthetic peptide directed towards the middle region of human NEDD4L |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEDD4L |
|---|---|
| Gene Synonyms | FLJ33870 KIAA0439 RSP5 hNedd4-2 NEDD4-2 NEDD4.2 hNEDD4-2 |
| Gene Full Name | Neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase |
| Gene Summary | NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK. |
| Alternative Names | FLJ33870 KIAA0439 RSP5 hNedd4-2 NEDD4-2 NEDD4.2 hNEDD4-2 |
| Molecular Weight(MW) | 110kDa |
| Sequence | 955 amino acids |
Western blot
Western blot
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: PANC1 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
