Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HT1080 cell lysate| Product Name | Rabbit anti-HERC5 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CEB1, CEBP1 |
| Immunogen | The immunogen for anti-HERC5 antibody: synthetic peptide directed towards the N terminal of human HERC5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HERC5 |
|---|---|
| Gene Synonyms | CEB1 CEBP1 |
| Gene Full Name | HECT and RLD domain containing E3 ubiquitin protein ligase 5 |
| Gene Summary | HERC5 is a probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. |
| Alternative Names | CEB1 CEBP1 |
| Molecular Weight(MW) | 117kDa |
| Sequence | 1024 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HT1080 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
