Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: DU145 cell lysate| Product Name | Rabbit anti-SH3RF1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ21602, KIAA1494, POSH, RNF142, SH3MD2 |
| Immunogen | The immunogen for anti-SH3RF1 antibody: synthetic peptide directed towards the middle region of human SH3RF1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHorseMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SH3RF1 |
|---|---|
| Gene Synonyms | FLJ21602 KIAA1494 POSH RNF142 SH3MD2 |
| Gene Full Name | SH3 domain containing ring finger 1 |
| Gene Summary | SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. |
| Alternative Names | FLJ21602 KIAA1494 POSH RNF142 SH3MD2 |
| Molecular Weight(MW) | 93kDa |
| Sequence | 888 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: DU145 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
