Immunohistochemistry
Immunohistochemistry with Human Colon lysate tissue at an antibody concentration of 5.0ug/ml using anti-ABCA5 antibodyWestern blot
1 ug/mlPositive Control: MCF-7 whole cell lysates| Product Name | Rabbit anti-ABCA5 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABC13, DKFZp451F117, DKFZp779N2435, EST90625, FLJ16381 |
| Immunogen | The immunogen for anti-ABCA5 antibody: synthetic peptide directed towards the C terminal of human ABCA5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCA5 |
|---|---|
| Gene Synonyms | ABC13 DKFZp451F117 DKFZp779N2435 EST90625 FLJ16381 |
| Gene Full Name | ATP-binding cassette, sub-family A (ABC1), member 5 |
| Gene Summary | The membrane-associated protein ABCA5 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). ABCA5 is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This gene is clustered among 4 other ABC1 family members on 17q24, but neither the substrate nor the function of this gene is known. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This encoded protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This gene is clustered among 4 other ABC1 family members on 17q24, but neither the substrate nor the function of this gene is known. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. |
| Alternative Names | ABC13 DKFZp451F117 DKFZp779N2435 EST90625 FLJ16381 |
| Molecular Weight(MW) | 186kDa |
| Sequence | 1642 amino acids |
Immunohistochemistry
Immunohistochemistry with Human Colon lysate tissue at an antibody concentration of 5.0ug/ml using anti-ABCA5 antibody
Western blot
1 ug/mlPositive Control: MCF-7 whole cell lysates| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
