Western blot
0.2-1 ug/mlPositive Control: OVCAR-3 cell lysate| Product Name | Rabbit anti-B4GALT2 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B4Gal-T2, B4Gal-T3, beta4Gal-T2 |
| Immunogen | The immunogen for anti-B4GALT2 antibody: synthetic peptide directed towards the middle region of human B4GALT2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | B4GALT2 |
|---|---|
| Gene Synonyms | B4Gal-T2 B4Gal-T3 beta4Gal-T2 |
| Gene Full Name | UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2 |
| Gene Summary | This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Two transcript variants encoding the same protein have been found for this gene. |
| Alternative Names | B4Gal-T2 B4Gal-T3 beta4Gal-T2 |
| Molecular Weight(MW) | 42kDa |
| Sequence | 372 amino acids |
| Entrez Gene | 8704 |
|---|---|
| SwissProt ID | O60909 |
| Protein Accession | NP_001005417 |
Western blot
0.2-1 ug/mlPositive Control: OVCAR-3 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
