Immunoblot
1: SREC pulldown from lysate from 10^6 human 293T cellsPrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-Alexa FluorSecondary Antibody Dilution: 1:5000Gene Name: WDR33Submitted by: AnonymousWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-WDR33 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ11294, WDC146, NET14 |
| Immunogen | The immunogen for anti-WDR33 antibody: synthetic peptide directed towards the middle region of human WDR33 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePigRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WDR33 |
|---|---|
| Gene Synonyms | FLJ11294 WDC146 NET14 |
| Gene Full Name | WD repeat domain 33 |
| Gene Summary | WDR33 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is highly expressed in testis and the protein is localized to the nucleus. This gene may play important roles in the mechanisms of cytodifferentiation and/or DNA recombination.This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is highly expressed in testis and the protein is localized to the nucleus. This gene may play important roles in the mechanisms of cytodifferentiation and/or DNA recombination. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Alternative Names | FLJ11294 WDC146 NET14 |
| Molecular Weight(MW) | 30kDa |
| Sequence | 257 amino acids |
| Entrez Gene | 55339 |
|---|---|
| SwissProt ID | Q6NUQ0 |
| Protein Accession | NP_001006624 |
Immunoblot
1: SREC pulldown from lysate from 10^6 human 293T cellsPrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-Alexa FluorSecondary Antibody Dilution: 1:5000Gene Name: WDR33Submitted by: Anonymous
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
