Western blot
Positive Control: Lane1: 50ug RCC4 lysate, Lane2: 50ug RCC4 lysate, Lane3: 50ug 786-0 lysate, Lane4: 50ug 786-0 lysatePrimary Antibody Dilution : 1:1000Secondary Antibody : Anti-rabbit-Alexa 680 Secondry Antibody Dilution : 1:5000Submitted by: Dr Ester Hammond, Gray Institute for Radiation Oncology and Biology-Department of Oncology, University of OxfordWestern blot
0.2-1 ug/mlPositive Control: Jurkat cell lysate| Product Name | Rabbit anti-MPG polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AAG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg, ADPG |
| Immunogen | The immunogen for anti-MPG antibody: synthetic peptide directed towards the C terminal of human MPG |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MPG |
|---|---|
| Gene Synonyms | AAG APNG CRA36.1 MDG Mid1 PIG11 PIG16 anpg ADPG |
| Gene Full Name | N-methylpurine-DNA glycosylase |
| Gene Summary | MPG functions in the hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. |
| Alternative Names | AAG APNG CRA36.1 MDG Mid1 PIG11 PIG16 anpg ADPG |
| Molecular Weight(MW) | 32kDa |
| Sequence | 293 amino acids |
| Entrez Gene | 4350 |
|---|---|
| SwissProt ID | Q5J9I4 |
| Protein Accession | NP_001015052 |
Western blot
Positive Control: Lane1: 50ug RCC4 lysate, Lane2: 50ug RCC4 lysate, Lane3: 50ug 786-0 lysate, Lane4: 50ug 786-0 lysatePrimary Antibody Dilution : 1:1000Secondary Antibody : Anti-rabbit-Alexa 680 Secondry Antibody Dilution : 1:5000Submitted by: Dr Ester Hammond, Gray Institute for Radiation Oncology and Biology-Department of Oncology, University of Oxford
Western blot
0.2-1 ug/mlPositive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
