MPG functions in the hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.1) Oh, J.H., (2005) Mamm. Genome 16 (12), 942-954.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MPG.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
MPG antibody can be used for detection of MPG by ELISA at 1:62500. MPG antibody can be used for detection of MPG by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on RCC4, 786-0 at 1:1000 (Lane1: 50ug RCC4 lysate, Lane2: 50ug RCC4 lysate, Lane3: 50ug 786-0 lysate, Lane4: 50ug 786-0 lysate).
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
MPG antibody can be used for detection of MPG by ELISA at 1:62500. MPG antibody can be used for detection of MPG by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MPG antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.