Immunohistochemical
ImmunohistochemicalWestern blot
CSTB antibody - middle region validated by WB using Transfected 293T cell lysate at 0.25ug/ml.| Product Name | Rabbit anti-CSTB polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CST6, EPM1, PME, STFB, ULD, EPM1A |
| Immunogen | The immunogen for anti-CSTB antibody: synthetic peptide directed towards the middle region of human CSTB |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CSTB |
|---|---|
| Gene Synonyms | CST6 EPM1 PME STFB ULD EPM1A |
| Gene Full Name | Cystatin B (stefin B) |
| Gene Summary | CSTB is a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in CSTB gene are responsible for the primary defects in patients with progressive myoclonic epilepsy a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy.The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). |
| Alternative Names | CST6 EPM1 PME STFB ULD EPM1A |
| Molecular Weight(MW) | 11kDa |
| Sequence | 98 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
CSTB antibody - middle region validated by WB using Transfected 293T cell lysate at 0.25ug/ml.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
