Immunohistochemical
ImmunohistochemicalWestern blot
5% MilkELISA Titer: dilution: 1:500Positive Control: Mouse Brain lysateWestern blot
Lane 1: 5ug in vitro translation without DNA Lane 2: 5ug in vitro translation hNeur1-pcDNA3 Lane 3: 60ug in vitro translation rNeur1-pcDNA3 Lane 4: 60ug HEK293 cell lysate (not transfected) Lane 5: 60ug hNeur1-pcDNA3 overexpressed in HEK293 cells Lane 6: 60ug rNeur1-pcDNA3 overexpressed in HEK293 cells Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:5000 Color/Signal Descriptions:PDE9A Gene Name:Kati Taal and Richard Tamme, Department of Gene Technology, Tallinn University of Technology. Submitted by:Western blot
1.0 ug/mlPositive Control: Jurkat cell lysate| Product Name | Rabbit anti-PDE9A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPDE9A2 |
| Immunogen | The immunogen for anti-PDE9A antibody: synthetic peptide directed towards the N terminal of human PDE9A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDE9A |
|---|---|
| Gene Synonyms | HSPDE9A2 |
| Gene Full Name | Phosphodiesterase 9A |
| Gene Summary | PDE9A catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides.The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. |
| Alternative Names | HSPDE9A2 |
| Molecular Weight(MW) | 61kDa |
| Sequence | 526 amino acids |
| Entrez Gene | 5152 |
|---|---|
| SwissProt ID | O76083-13 |
| Protein Accession | NP_001001581 |
Immunohistochemical
Immunohistochemical
Western blot
5% MilkELISA Titer: dilution: 1:500Positive Control: Mouse Brain lysate
Western blot
Lane 1: 5ug in vitro translation without DNA Lane 2: 5ug in vitro translation hNeur1-pcDNA3 Lane 3: 60ug in vitro translation rNeur1-pcDNA3 Lane 4: 60ug HEK293 cell lysate (not transfected) Lane 5: 60ug hNeur1-pcDNA3 overexpressed in HEK293 cells Lane 6: 60ug rNeur1-pcDNA3 overexpressed in HEK293 cells Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:5000 Color/Signal Descriptions:PDE9A Gene Name:Kati Taal and Richard Tamme, Department of Gene Technology, Tallinn University of Technology. Submitted by:
Western blot
1.0 ug/mlPositive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
