Immunohistochemistry
Researcher: Received from anonymousApplication: IHCSpecies+tissue/cell type:THP-1 derived macrophage activated with iMtbPrimary antibody dilution: 1:200Secondary antibody: Goat anti-rabbit Alexa Fluor 647Secondary antibody dilution:1:333Immunohistochemistry
Researcher: Received from anonymousApplication: IHCSpecies+tissue/cell type:THP-1 derived macrophagePrimary antibody dilution: 1:200Secondary antibody: Goat anti-rabbit Alexa Fluor 647Secondary antibody dilution:1:333| Product Name | Rabbit anti-ACSL3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACS3, FACL3, PRO2194 |
| Immunogen | The immunogen for anti-ACSL3 antibody: synthetic peptide directed towards the N terminal of human ACSL3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Species Reactivity | BovineHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACSL3 |
|---|---|
| Gene Synonyms | ACS3 FACL3 PRO2194 |
| Gene Full Name | Acyl-CoA synthetase long-chain family member 3 |
| Gene Summary | ACSL3 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. |
| Alternative Names | ACS3 FACL3 PRO2194 |
| Molecular Weight(MW) | 80kDa |
| Sequence | 720 amino acids |
Immunohistochemistry
Researcher: Received from anonymousApplication: IHCSpecies+tissue/cell type:THP-1 derived macrophage activated with iMtbPrimary antibody dilution: 1:200Secondary antibody: Goat anti-rabbit Alexa Fluor 647Secondary antibody dilution:1:333
Immunohistochemistry
Researcher: Received from anonymousApplication: IHCSpecies+tissue/cell type:THP-1 derived macrophagePrimary antibody dilution: 1:200Secondary antibody: Goat anti-rabbit Alexa Fluor 647Secondary antibody dilution:1:333| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
