Immunoblot
1. A431 cell lysate + control siRNA 2. A431 cell lysate + SEMA4F siRNAPrimary Antibody Dilution: 1:600Secondary Antibody: Donkey Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: SEMA4FSubmitted by: Dr. Tianliang Sun, Max Planck Institute for Heart and Lung ResearchImmunoblot
1. Untransfected MIA-PACA2 cell lysate2. Untransfected MIA-PACA2 cell lysate3. Untransfected MIA-PACA2 cell lysate4. hSEMA4F transfected MIA-PACA2 cell lysate5. Untransfected MIA-PACA2 cell lysate6. hSEMA4F transfected MIA-PACA2 cell lysatePrimary Antibody Dilution: 1:600Secondary Antibody: Donkey Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: SEMA4FSubmitted by: Dr. Tianliang Sun, Max Planck Institute for Heart and Lung ResearchWestern blot
human cell line A431Primary antibody dilution and incubation time:1:600, 4 degree overnightSecondary antibody used and dilution and incubation time: 1:3000, RT 2 hoursWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Transfected 293T| Product Name | Rabbit anti-SEMA4F polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SEMAM, SEMAW, M-SEMA, PRO2353, m-Sema-M |
| Immunogen | The immunogen for anti-SEMA4F antibody: synthetic peptide directed towards the n terminal of human SEMA4F |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SEMA4F |
|---|---|
| Gene Synonyms | SEMAM SEMAW M-SEMA PRO2353 m-Sema-M |
| Gene Full Name | Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F |
| Gene Summary | SEMA4F has growth cone collapse activity against retinal ganglion-cell axons. |
| Alternative Names | SEMAM SEMAW M-SEMA PRO2353 m-Sema-M |
| Molecular Weight(MW) | 67kDa |
| Sequence | 615 amino acids |
Immunoblot
1. A431 cell lysate + control siRNA 2. A431 cell lysate + SEMA4F siRNAPrimary Antibody Dilution: 1:600Secondary Antibody: Donkey Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: SEMA4FSubmitted by: Dr. Tianliang Sun, Max Planck Institute for Heart and Lung Research
Immunoblot
1. Untransfected MIA-PACA2 cell lysate2. Untransfected MIA-PACA2 cell lysate3. Untransfected MIA-PACA2 cell lysate4. hSEMA4F transfected MIA-PACA2 cell lysate5. Untransfected MIA-PACA2 cell lysate6. hSEMA4F transfected MIA-PACA2 cell lysatePrimary Antibody Dilution: 1:600Secondary Antibody: Donkey Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: SEMA4FSubmitted by: Dr. Tianliang Sun, Max Planck Institute for Heart and Lung Research
Western blot
human cell line A431Primary antibody dilution and incubation time:1:600, 4 degree overnightSecondary antibody used and dilution and incubation time: 1:3000, RT 2 hours
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Transfected 293T| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
